Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis
S Inouye - Protein expression and purification, 2007 - Elsevier
The Ca2+-triggered luciferin-binding protein of Renilla reniformis (RLBP) is a non-covalent
complex of apoprotein (apoRLBP) and coelenterazine (luciferin). The gene encoding
apoRLBP with 552 nucleotides has been synthesized by assembly PCR methods with
synthetic oligonucleotides, and the histidine-tagged apoRLBP expressed as a soluble form
in the periplasmic space of Escherichia coli cells. The apoRLBP was purified by nickel
chelate chromatography and the procedure yielded 18.2 mg of recombinant apoRLBP from …
complex of apoprotein (apoRLBP) and coelenterazine (luciferin). The gene encoding
apoRLBP with 552 nucleotides has been synthesized by assembly PCR methods with
synthetic oligonucleotides, and the histidine-tagged apoRLBP expressed as a soluble form
in the periplasmic space of Escherichia coli cells. The apoRLBP was purified by nickel
chelate chromatography and the procedure yielded 18.2 mg of recombinant apoRLBP from …
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